Factors
to Consider When Applying
Serum Method to Stool
•Amount
sample is diluted prior to analysis
•Technique/amount
of washing of ELISA plates
–Greater
solid contaminant of fecal fluid vs. serum
•Mathematical
conversion of OD to a Unit
•How
calculated Unit is interpreted: norm vs.
abnorm
•Centrifuge
speed for stool (too high neg
result)
•Proper
collection and preservation of stool
